Zetterström T, Vernet L, Ungerstedt U, Tossman U, Jonzon B, Fredholm B B
Neurosci Lett. 1982 Apr 16;29(2):111-5. doi: 10.1016/0304-3940(82)90338-x.
A thin dialysis tube was implanted stereotaxically under halothane anesthesia in the caudate nucleus of Sprague-Dawley rats and perfused with Ringer solution at a rate of 2 microliters/min. Initially there was a high rate of purine outflow but after 1-2 h of perfusion the rate was essentially constant (anesthetized - adenosine 0.4 +/- 0.04 microM, inosine 0.8 +/- 0.2 microM; non-anesthetized - adenosine 0.33 +/- 0.03 microM, inosine 0.21 +/- 0.07 microM). Hypoxia (9% O2) increased the levels more than 3-fold. The adenosine deaminase inhibitor erythro-2-(2-hydroxy-3-nonyl)adenine (EHNA) increased the adenosine level and decreased the inosine level. In vitro recovery of adenosine was about 30%. Therefore, we conclude that the free exchangable concentration of adenosine in the rat brain is likely to be 102 micro M. This level is high enough to potentially affect central nervous function.
在氟烷麻醉下，将一根细透析管立体定向植入Sprague-Dawley大鼠的尾状核，并以2微升/分钟的速度用林格溶液灌注。最初嘌呤流出率很高，但灌注1-2小时后，流出率基本恒定（麻醉状态下 - 腺苷0.4±0.04微摩尔，肌苷0.8±0.2微摩尔；未麻醉状态下 - 腺苷0.33±0.03微摩尔，肌苷0.21±0.07微摩尔）。低氧（9%氧气）使水平增加超过3倍。腺苷脱氨酶抑制剂erythro-2-(2-羟基-3-壬基)腺嘌呤（EHNA）增加了腺苷水平并降低了肌苷水平。腺苷的体外回收率约为30%。因此，我们得出结论，大鼠脑中腺苷的游离可交换浓度可能为102微摩尔。这个水平足够高，有可能影响中枢神经功能。
