Nikiforov A A
Tsitologiia. 1982 Apr;24(4):449-55.
At 20 degrees when active transport of an organic acid - fluorescein in superficial proximal tubules of surviving rat kidney does not depend on the external Na, the capability of acetate (as well as succinate and D,L-leucine) of stimulating the fluorescein uptake in the tubules was studied. As it turned out, acetate and leucine stimulated the fluorescein uptake at 120 mM of Na in the bath medium but had no effect at 10 mM of Na. Succinate has a biphasic effect on the fluorescein transport (stimulation at low succinate concentration), which does not depend on Na concentration in the medium. The effect of acetate disappears with the presence of ouabain in the medium. The stimulation of fluorescein uptake by acetate is accompanied by somewhat increased degree of reduction of cellular pyridine nucleotides. Pyridine nucleotides are reduced more markedly in the presence of ouabain or in the absence of Na from the bath medium. The disappearance of acetate effect on the fluorescein transport is explained by the fact of transition of cell mitochondria in the inactive state 4, due to the decrease in the level of cellular ADP when Na, K-ATPase is inhibited by ouabain or by diminution of tissue Na+ concentration.
在20摄氏度时，存活大鼠肾脏浅表近端小管中有机酸荧光素的主动转运不依赖于细胞外钠离子，在此条件下研究了乙酸盐（以及琥珀酸盐和D，L-亮氨酸）刺激小管中荧光素摄取的能力。结果表明，在浴液中钠离子浓度为120 mM时，乙酸盐和亮氨酸刺激荧光素摄取，但在钠离子浓度为10 mM时则无作用。琥珀酸盐对荧光素转运具有双相效应（在低琥珀酸盐浓度下刺激），且该效应不依赖于介质中的钠离子浓度。介质中存在哇巴因时，乙酸盐的效应消失。乙酸盐对荧光素摄取的刺激伴随着细胞吡啶核苷酸还原程度的略有增加。在存在哇巴因或浴液中无钠离子的情况下，吡啶核苷酸的还原更为明显。乙酸盐对荧光素转运的效应消失可解释为：当哇巴因抑制钠钾ATP酶或组织钠离子浓度降低导致细胞ADP水平下降时，细胞线粒体转变为非活性状态4。
