Pasyk S, Schaper W, Schaper J, Pasyk K, Miskiewicz G, Steinseifer B
Am J Physiol. 1982 Jun;242(6):H1031-7. doi: 10.1152/ajpheart.1982.242.6.H1031.
With [3H]thymidine, DNA synthetic activity in coronary collateral arterioles was assessed in dogs subjected to progressive stenosis of the left circumflex coronary artery for different periods (36 h-5 days). Coronary flow, reactive hyperemia, and pressure gradient (aortic pressure, peripheral coronary pressure) were measured. Normal animals and animals with sham operations served as controls. Labeling of nuclei was seen in seven of the constricted dogs. Proliferative activity was highest at the level of the smallest diameters of the collateral vessels (midzone). There was heavy labeling in intima and media. Adventitial and myocardial mesenchymal cells also incorporated [3H]thymidine. No radioactive DNA was found in control animals. The data suggest that the active growth process in collaterals after constriction of the left circumflex coronary artery appears early, as there is evidence of collateral growth after 36 h of constriction. Peak growth as evidenced by [3H]thymidine incorporation was reached when the occlusion period was 4 days.
采用[3H]胸腺嘧啶核苷，对不同时段（36小时至5天）接受左旋冠状动脉渐进性狭窄的犬的冠状动脉侧支小动脉中的DNA合成活性进行评估。测量冠状动脉血流量、反应性充血和压力梯度（主动脉压力、外周冠状动脉压力）。正常动物和接受假手术的动物作为对照。在7只狭窄犬中观察到细胞核标记。增殖活性在侧支血管最小直径水平（中区）最高。内膜和中膜有大量标记。外膜和心肌间充质细胞也掺入了[3H]胸腺嘧啶核苷。对照动物中未发现放射性DNA。数据表明，左旋冠状动脉狭窄后侧支的活跃生长过程出现较早，因为在狭窄36小时后就有侧支生长的证据。当闭塞期为4天时，[3H]胸腺嘧啶核苷掺入所证明的生长达到峰值。
