Flumerfelt B A, Contestabile A
Anat Embryol (Berl). 1982;163(4):435-46. doi: 10.1007/BF00305557.
The histochemical distribution of acetylcholinesterase (AChE) was studied in the habenulo-interpeduncular pathway of normal rats and after electrolytic and kainic acid lesions of the habenular nuclei. From these combined observations it appears that the AChE-rich projection to the interpeduncular nucleus derives from both the medial and the lateral habenular nuclei. The lateral nucleus of the habenula is the main source of AChE-rich fibres in the fasciculus retroflexus, and a number of stained fibres also derive from the stria medullaris. While total habenular lesion completely deprived the fasciculus retroflexus of AChE-stained fibres, a direct effect on the enzyme distribution in the interpeduncular nucleus was only apparent as its rostral pole. In the remainder of the nucleus the AChE distribution did not undergo obvious changes in comparison with the normal pattern, except for a moderate decrease in overall reaction intensity in cases with subtotal habenular lesion bilaterally. The above results are consistent with the observation derived from experiments involving kainic acid injection into the habenula. The neurotoxic effect of kainic acid was highly selective for specific types of neurons in the lateral habenula, while the neurons of the medial habenula were completely unaffected. The existence of an AChE-rich projection from the lateral habenula to the interpeduncular nucleus was supported by a corresponding decrease in enzyme activity in the lateral habenula and fasciculus retroflexus after kainic acid treatment.
研究了正常大鼠以及在缰核进行电解损伤和 kainic 酸损伤后，乙酰胆碱酯酶（AChE）在缰核 - 脚间通路中的组织化学分布。综合这些观察结果表明，向脚间核的富含 AChE 的投射源自内侧和外侧缰核。缰核的外侧核是缰核后束中富含 AChE 纤维的主要来源，并且一些染色纤维也源自髓纹。虽然完全的缰核损伤使缰核后束完全失去了 AChE 染色纤维，但对脚间核中酶分布的直接影响仅在其嘴侧极明显。在核的其余部分，与正常模式相比，AChE 分布没有明显变化，只是在双侧缰核部分损伤的情况下，总体反应强度有适度降低。上述结果与向缰核注射 kainic 酸的实验观察结果一致。Kainic 酸的神经毒性作用对外侧缰核中的特定类型神经元具有高度选择性，而内侧缰核的神经元则完全不受影响。在 kainic 酸处理后，外侧缰核和缰核后束中酶活性相应降低，这支持了从外侧缰核到脚间核存在富含 AChE 的投射。
