Puig M M, Laorden M L, Miralles F S, Olaso M J
Anesthesiology. 1982 Jul;57(1):1-4. doi: 10.1097/00000542-198207000-00001.
The authors measured endorphin levels in the cerebrospinal fluid (CSF) of 12 patients with chronic pain due to lumbar disc syndrome and eight patients with acute postoperative pain. These were compared with CSF endorphin levels in 20 control patients with no history of pain. Endorphins were extracted by adsorption to a synthetic resin (Amberlite XAD-2), eluted with methanol, and assayed using the electrically stimulated mouse vas deferens. Results were expressed as methionine-enkephalin (Met-E) equivalents, which was the standard in the bioassay. The CSF endorphin level was 0.42 +/- 0.07 pmol/ml (mean +/- SE) in the postoperative group, 1.44 +/- 0.2 pmol/ml in the chronic pain group, and 4.36 +/- 0.89 pmol/ml in the control group. CSF endorphin levels in the two pain groups differed significantly from both the control group and each other. These results suggest a correlation between pain levels and endorphin concentration in the CSF; however, in the acute postoperative pain group the influence of other factors such as anesthesia or surgical stress cannot be evaluated.
作者测量了12例因腰椎间盘综合征导致慢性疼痛的患者以及8例术后急性疼痛患者脑脊液（CSF）中的内啡肽水平。并将这些结果与20例无疼痛史的对照患者的脑脊液内啡肽水平进行了比较。内啡肽通过吸附到合成树脂（Amberlite XAD - 2）上进行提取，用甲醇洗脱，然后使用电刺激的小鼠输精管进行测定。结果以甲硫氨酸脑啡肽（Met - E）当量表示，这是生物测定中的标准。术后组脑脊液内啡肽水平为0.42±0.07 pmol/ml（平均值±标准误），慢性疼痛组为1.44±0.2 pmol/ml，对照组为4.36±0.89 pmol/ml。两个疼痛组的脑脊液内啡肽水平与对照组以及两组之间均存在显著差异。这些结果表明疼痛程度与脑脊液内啡肽浓度之间存在相关性；然而，在术后急性疼痛组中，无法评估麻醉或手术应激等其他因素的影响。
