Cheevers W P, Roberson S M, Brassfield A L, Davis W C, Crawford T B
Am J Vet Res. 1982 May;43(5):804-6.
A virus with the morphologic and biochemical properties of the family Retroviridae has been isolated from cultured cells explanted from a malignant tumor induced by intradermal inoculation of equine sarcoid cells into a combined immunodeficient Arabian foal. By electron microscopy, intracytoplasmic, extracellular, and budding particles measuring 89 to 120 nm with electron-lucent cores were seen. Virus purified from the medium of cultured cells had a buoyant density of 1.15 g/cm3 in isopycnic sucrose-gradient centrifugation, incorporated radiolabeled uridine but not thymidine, and had constitutive RNA-dependent DNA polymerase which required Mn2+ for optimal endogenous activity.
从一匹联合免疫缺陷阿拉伯幼驹体内通过皮内接种马肉瘤细胞诱导产生的恶性肿瘤中分离出的培养细胞中，分离出了一种具有逆转录病毒科形态学和生化特性的病毒。通过电子显微镜观察，可见胞质内、细胞外以及出芽的颗粒，其大小为89至120纳米，核心电子密度较低。从培养细胞培养基中纯化的病毒在等密度蔗糖梯度离心中的浮力密度为1.15 g/cm³，能掺入放射性标记的尿苷但不能掺入胸苷，并且具有组成型RNA依赖性DNA聚合酶，该酶需要Mn²⁺才能达到最佳内源性活性。
