Iritani Y, Katagiri K, Arita H
Am J Vet Res. 1980 Dec;41(12):2114-8.
Hemagglutinin (HA) of Haemophilus paragallinarum was purified, and its immunologic, chemical, and morphologic properties were studied. The HA was purified by gel filtration on Sepharose 6B and by subsequent ultracentrifugation of trypsinized cells after pretreatment with neuraminidase. After gel filtration, the HA component (fractioned HA) gave 1 precipitin line by gel diffusion, and after inoculation in chickens, hemagglutinination-inhibition antibody to trypsin-sensitive HA antigen was found. Chickens inoculated with the fractionated HA were protected from challenge exposure with H paragallinarum. In a sample further purified by ultracentrifugation (purified HA), a single protein band was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; it had a molecular weight of approximately 39,000. A molecular weight of several hundred thousand to several million was observed by gel filtration. Seemingly, HA of H paragallinarum is an aggregate of more than 20 HA sub-units. The purified HA included protein and some sugars, and according to electron microscopic observation, had a filamentous structure.
对副鸡嗜血杆菌的血凝素（HA）进行了纯化，并研究了其免疫学、化学和形态学特性。通过在Sepharose 6B上进行凝胶过滤以及随后用神经氨酸酶预处理后对胰蛋白酶处理的细胞进行超速离心来纯化HA。凝胶过滤后，HA成分（分级分离的HA）通过凝胶扩散产生1条沉淀线，接种鸡后，发现了对胰蛋白酶敏感的HA抗原的血凝抑制抗体。接种分级分离的HA的鸡受到保护，免受副鸡嗜血杆菌的攻击暴露。在通过超速离心进一步纯化的样品（纯化的HA）中，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳检测到一条单一的蛋白带；其分子量约为39,000。通过凝胶过滤观察到分子量为几十万到几百万。显然，副鸡嗜血杆菌的HA是20多个HA亚基的聚集体。纯化的HA含有蛋白质和一些糖类，根据电子显微镜观察，具有丝状结构。
