[通过Ficoll-Paque梯度分离的单核细胞悬液中酯酶阳性细胞的快速鉴定方法]
[Rapid method for the identification of esterase-positive cells in suspensions of mononuclear cells separated by a Ficoll-Paque gradient].
作者信息
Russo M, Pizzella T, Nardiello S, Galanti B
出版信息
Boll Soc Ital Biol Sper. 1982 Apr 30;58(8):462-6.
Cytochemical staining for acid alpha-naphthyl acetate esterase (ANAE) activity is widely used for identifying monocytes in mixed cell populations; esterase staining is usually performed on slide prepartions of whole blood, buffy coat or Ficoll-Paque separated mononuclear cells (MC). In this paper we propose a method for staining MC while they are in suspension, which utilizes, with slight modifications, the same reagents used in the procedure for esterase staining on smears. Suspension method is rapid and costless; furthermore, it overcomes the loss of esterase stainability observed in slide preparations of Ficoll-Paque separated MC.U
酸性α-萘乙酸酯酶（ANAE）活性的细胞化学染色广泛用于在混合细胞群体中鉴定单核细胞；酯酶染色通常在全血、血沉棕黄层或经Ficoll-Paque分离的单核细胞（MC）的玻片涂片上进行。在本文中，我们提出了一种在MC处于悬浮状态时进行染色的方法，该方法稍加修改后利用了涂片酯酶染色程序中使用的相同试剂。悬浮法快速且成本低；此外，它克服了在经Ficoll-Paque分离的MC的玻片制备中观察到的酯酶染色性丧失的问题。
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