Sasagawa T, Ericsson L H, Walsh K A, Schreiber W E, Fischer E H, Titani K
Biochemistry. 1982 May 11;21(10):2565-9. doi: 10.1021/bi00539a041.
The complete amino acid sequence of calmodulin from human brain has been determined by using peptides derived from digests with trypsin. Staphylococcus aureus V8 protease, and cyanogen bromide. The peptides were purified by means of reversed-phase high-performance liquid chromatography and analyzed with a sequenator. The protein contains 148 amino acid residues and has a molecular weight of 16792. As in other calmodulins, the amino-terminal residue of the protein is blocked by an acetyl group, and a trimethyllysine residue is located at position 115. The only difference between this sequence and those fully determined in other species is the assignment of amide groups.
人脑钙调蛋白的完整氨基酸序列已通过对胰蛋白酶、金黄色葡萄球菌V8蛋白酶和溴化氰消化产物衍生的肽段进行测定而确定。这些肽段通过反相高效液相色谱法进行纯化，并使用序列分析仪进行分析。该蛋白质含有148个氨基酸残基，分子量为16792。与其他钙调蛋白一样，该蛋白质的氨基末端残基被乙酰基封闭，一个三甲基赖氨酸残基位于第115位。此序列与其他物种已完全确定的序列之间的唯一差异在于酰胺基团的归属。
