Gimenez-Gallego G, Conn G, Hatcher V B, Thomas K A
Biochem Biophys Res Commun. 1986 Jul 31;138(2):611-7. doi: 10.1016/s0006-291x(86)80540-x.
Acidic fibroblast growth factor is a potent mitogen for a variety of cells in culture, including vascular endothelial cells, and is angiogenic in vivo. The complete amino acid sequence of human brain-derived acidic fibroblast growth factor has been determined from amino terminal sequence analysis and carboxypeptidase A digestion of the whole protein and sequence analyses of peptides generated by tryptic, Staphylococcus aureus V8 protease and cyanogen bromide cleavages. A potential Asn-Gly-Ser glycosylation sequence is present in the human protein. The complete amino acid sequence is compared to that of the equivalent protein purified from bovine brain.
酸性成纤维细胞生长因子是培养中的多种细胞（包括血管内皮细胞）的一种强效有丝分裂原，并且在体内具有血管生成作用。人脑源性酸性成纤维细胞生长因子的完整氨基酸序列已通过对整个蛋白质进行氨基末端序列分析和羧肽酶A消化以及对胰蛋白酶、金黄色葡萄球菌V8蛋白酶和溴化氰裂解产生的肽段进行序列分析来确定。人蛋白质中存在一个潜在的天冬酰胺-甘氨酸-丝氨酸糖基化序列。将完整的氨基酸序列与人脑来源的相应蛋白质的氨基酸序列进行了比较。
