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[红细胞膜内ATP含量及蛋白质结构状态]

[Content of intracellular ATP and structural state of proteins in the erythrocyte membrane].

作者信息

Slobozhanina E I, Chernitskiĭ E A, Koslova N M

出版信息

Biofizika. 1982 May-Jun;27(3):425-9.

PMID:7093324
Abstract

Effects of depletion and resynthesis of intracellular ATP on the properties of human erythrocytes (deformability, osmotic fragility) and on the structural parameters of their membranes (U. V. fluorescence spectra, protein aggregation) were studied. Incubation of the erythrocytes in the absence of glucose at 37 degrees C was shown to result in a number of consequent alterations: crenating and decrease in deformability of erythrocyte cells--a change in relative contents of membrane proteins--aggregation of membrane proteins and reversible shift of their fluorescence spectra. The evidence is considered in favour of the spectra shift due to dephosphorylation of membrane proteins.

摘要

研究了细胞内ATP耗竭与再合成对人红细胞特性(变形性、渗透脆性)及其膜结构参数(紫外荧光光谱、蛋白质聚集)的影响。结果表明,红细胞在37℃无葡萄糖条件下孵育会导致一系列后续变化:红细胞皱缩且变形性降低——膜蛋白相对含量改变——膜蛋白聚集及其荧光光谱的可逆性移位。有证据支持膜蛋白去磷酸化导致光谱移位这一观点。

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1
[Content of intracellular ATP and structural state of proteins in the erythrocyte membrane].[红细胞膜内ATP含量及蛋白质结构状态]
Biofizika. 1982 May-Jun;27(3):425-9.
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