Nakahata T, Spicer S S, Cantey J R, Ogawa M
Blood. 1982 Aug;60(2):352-61.
When mouse marrow and spleen cells were cultured for over 12 days in methylcellulose containing media conditioned by pokeweed-mitogen-stimulated spleen cells, colonies containing mast cells and blast cells were observed. The characteristic morphology of the colonies and the time course of their development allowed in situ identification of the mast cell colonies. Identification of the mast cells was confirmed by metachromatic staining with toluidine blue and alcian blue, transmission electron microscopy, and by demonstration of the membrane receptors for IgE. Coculture studies with male and female marrow cells strongly indicated the single cell origin of individual colonies. Detailed cytologic analyses of mixed hemopoietic colonies and replating experiments of individual mixed hemopoietic and mast cell colonies clearly established the hemopoietic origin of mast cells. In replating experiments of individual mast cell colonies, those without blast cells did not yield secondary mast cell colonies. This result strongly indicated that morphologically recognizable mast cells have lost their self-renewing capabilities. The quantitative nature of the mast cell colony assay was supported by linearity studies and provides a method for studies of the progenitors of mouse mast cells.
当小鼠骨髓和脾细胞在含有经商陆有丝分裂原刺激的脾细胞条件培养液的甲基纤维素中培养超过12天时，观察到含有肥大细胞和原始细胞的集落。集落的特征形态及其发育的时间进程使得能够在原位鉴定肥大细胞集落。通过甲苯胺蓝和阿尔辛蓝的异染性染色、透射电子显微镜以及IgE膜受体的证明，证实了肥大细胞的鉴定。用雄性和雌性骨髓细胞进行的共培养研究有力地表明了单个集落的单细胞起源。对混合造血集落的详细细胞学分析以及单个混合造血和肥大细胞集落的再接种实验清楚地确定了肥大细胞的造血起源。在单个肥大细胞集落的再接种实验中，没有原始细胞的集落没有产生第二代肥大细胞集落。这一结果有力地表明，形态上可识别的肥大细胞已经失去了自我更新能力。肥大细胞集落测定的定量性质得到了线性研究的支持，并为小鼠肥大细胞祖细胞的研究提供了一种方法。
