Lew G M, Payer A, Quay W B
Cell Tissue Res. 1982;224(1):195-206. doi: 10.1007/BF00217279.
An ultrastructural and morphometric analysis was made of the nucleolar components in pinealocytes of 40 male Fischer rats sampled at eight times in an LD 12:12 photoperiod cycle. Comparisons of results from the eight times showed variation in estimated mean volume of the granular component of +/- 29%, and of the fibrillar component +/- 11%, in relation to daily means. Peaks in mean volume of total nucleolus and its granular component occurred at 1 h of light. Near maximal and minimal mean volumes of the fibrillar component both occurred during both light and dark. Fibrillar centers (nucleolar organizer regions) of different sizes were found at all sampling times. It is concluded that temporal patterns in 24-h changes in the nucleolar components are most prominent in the granular component, and are more complex than suggested by changes in total nucleolar size or mean dimensions, and than represented by a simple biphasic circadian rhythm. Examples of different stages in the migration of the granular component, and of possible sites of nucleo-cytoplasmic transfer of nucleolar material, are described.
对40只雄性Fischer大鼠松果体细胞中的核仁成分进行了超微结构和形态计量分析，这些大鼠在12:12的光照周期中于8个时间点取样。八次结果的比较显示，与每日平均值相比，颗粒成分的估计平均体积变化为±29%，纤维成分变化为±11%。核仁总体积及其颗粒成分的平均体积峰值出现在光照1小时时。纤维成分的平均体积接近最大值和最小值均出现在光照和黑暗期间。在所有取样时间均发现了不同大小的纤维中心（核仁组织区）。得出的结论是，核仁成分24小时变化的时间模式在颗粒成分中最为突出，且比核仁总体大小或平均尺寸变化所表明的更为复杂，也比简单的双相昼夜节律所呈现的更为复杂。描述了颗粒成分迁移不同阶段的实例以及核仁物质核质转移的可能位点。
