Benes L, Rotreklová E, Velcovský V, Pospísil M
Folia Biol (Praha). 1982;28(2):134-7.
The effect of gentamycin on bone marrow cells in unirradiated and irradiated mice was investigated by 3H-dTh incorporation into DNA using microautoradiography. Gentamycin was injected in vivo at 20 mg base/kg body weight at 8-h intervals during three days. In mice exposed to 4.78 Gy the injections of gentamycin were started on the 7th day after irradiation. In granulopoietic and erythropoietic cells the inhibition of entry of cells into S phase and the retardation of DNA replication was about 20%. The roughly 30% inhibition of entry of cells into S phase observed when the bone marrow cell population has been evaluated as a whole is discussed in terms of increased sensitivity of the young blast-like cells.
通过使用微放射自显影术检测³H-胸苷掺入DNA的情况，研究了庆大霉素对未受辐照和受辐照小鼠骨髓细胞的影响。在三天内，以20mg碱基/千克体重的剂量，每隔8小时对小鼠进行一次庆大霉素的体内注射。对于受到4.78Gy辐照的小鼠，在辐照后第7天开始注射庆大霉素。在粒细胞生成细胞和红细胞生成细胞中，细胞进入S期的抑制以及DNA复制的延迟约为20%。当将骨髓细胞群体作为一个整体进行评估时，观察到细胞进入S期受到约30%的抑制，这一点从年轻的母细胞样细胞敏感性增加的角度进行了讨论。
