Chayakul V, Reim M
Graefes Arch Clin Exp Ophthalmol. 1982;218(3):145-8. doi: 10.1007/BF02215652.
Alkali-burnt corneas of the rabbits with 1 N NaOH were studied periodically for enzymatic activities by biochemical methods. There was significant increase of aldolase (ALD) activity both in corneal epithelium and stroma 1, 2, 3 and 4 weeks after alkali burns. Glucose-6-phosphate dehydrogenase (G6PD) activity was significantly decreased in epithelium and was absent in stroma. Thus the breakdown of glucose would be present preferably in the Embden-Meyerhoff pathway instead of the pentose phosphate shunt. Lactate dehydrogenase (LDH) activity of corneal epithelium and stroma was significantly decreased 1, 2, 3, and 4 weeks after alkali burns and the possible pathway of glycolysis might channel to citric acid cycle, in which malate dehydrogenase (MDH) could indicate the important role in this pathway.
采用生化方法定期研究用1N氢氧化钠造成碱烧伤的兔角膜的酶活性。碱烧伤后1、2、3和4周，角膜上皮和基质中的醛缩酶（ALD）活性显著增加。葡萄糖-6-磷酸脱氢酶（G6PD）活性在上皮中显著降低，在基质中未检测到。因此，葡萄糖的分解可能主要通过糖酵解途径而非磷酸戊糖途径。碱烧伤后1、2、3和4周，角膜上皮和基质中的乳酸脱氢酶（LDH）活性显著降低，糖酵解的可能途径可能转向柠檬酸循环，其中苹果酸脱氢酶（MDH）可能在该途径中起重要作用。
