Burke J M, Foster S J
Graefes Arch Clin Exp Ophthalmol. 1982;218(3):153-5. doi: 10.1007/BF02215654.
Cultures of rabbit retinal pigment epithelium (RPE) were exposed to normal vitreous and to vitreous injured by intravitreal injection of foreign particles. Counts of labeled RPE nuclei after incubation with 3H-thymidine in vitro indicated an increase in DNA synthesis with exposure to normal vitreous and an even greater increase with exposure to injured vitreous. Fractionation of injured vitreous demonstrated that the apparent proliferation stimulus resided in the cell-free supernate. The data suggest that normal vitreous contains a humoral factor that stimulates RPE proliferation and that levels of an active agent increase after vitreal injury. RPE injected into the vitreous also responds by increased DNA synthesis to subsequent vitreal injury. This observation implies that foreign substances in the vitreous, as after vitreal hemorrhage, promote development of extraretinopathies involving RPE by stimulating intravitreal proliferation of invasive RPE cells.
将兔视网膜色素上皮（RPE）培养物分别暴露于正常玻璃体和经玻璃体内注射异物而受损的玻璃体中。体外与3H-胸腺嘧啶核苷孵育后，标记的RPE细胞核计数表明，暴露于正常玻璃体时DNA合成增加，而暴露于受损玻璃体时增加得更多。对受损玻璃体进行分级分离表明，明显的增殖刺激物存在于无细胞的上清液中。数据表明，正常玻璃体含有一种刺激RPE增殖的体液因子，并且玻璃体损伤后活性剂水平会升高。注入玻璃体的RPE对随后的玻璃体损伤也会通过增加DNA合成作出反应。这一观察结果意味着，玻璃体中的异物，如玻璃体出血后，通过刺激侵入性RPE细胞在玻璃体内增殖，促进涉及RPE的视网膜外病变的发展。
