Structural changes of human and monkey trabecular meshwork following in vitro cultivation.

The entire chamber angle tissue of ten monkey eyes and nine normal human eyes was cultivated in organ cultures for 1-10 days and then investigated by electron microscopy. We found that the uveal and corneoscleral trabecular cells often degenerate as early as 2-3 days after explantation, whereas the cells of the cribriform region proliferate and show an increasing number of cell organelles (mitochondria, endoplasmic reticulum and Golgi complexes). Regarding the behaviour in vitro, we distinguished three different cell populations in the trabecular meshwork with probably different functions: (1) the endothelial cells of Schlemm's canal, (2) the trabecular cells of the cribriform region and (3) the uveal or corneoscleral trabecular cells.