Bogusz M, Bialka J, Gierz J
Forensic Sci Int. 1982 Jul-Aug;20(1):27-33. doi: 10.1016/0379-0738(82)90102-5.
Samples of blood, liver and brain taken from 13 cases of fatal poisoning with barbiturates, antidepressants, phenothiazines and anticonvulsants were digested with trypsin and beta-glucuronidase. The concentrations of drugs were higher in hydrolyzed livers in barbiturate and phenothiazine poisonings. In the case of diazepam, phenytoin, carbamazepine, amitriptyline, imipramine and chloroprothixene poisonings the results obtained after enzymic hydrolysis were the same or worse than in controls. The blood levels of drugs in non-fatal poisonings with diazepam, doxepin and chlorpromazine were similar in samples digested with trypsin and in controls. All samples were extracted with Amberlite XAD-2 resin by XAD-2 Bag technique.
从13例因巴比妥类药物、抗抑郁药、吩噻嗪类药物和抗惊厥药致死中毒的病例中采集血液、肝脏和大脑样本，用胰蛋白酶和β-葡萄糖醛酸酶进行消化。在巴比妥类药物和吩噻嗪类药物中毒时，水解后的肝脏中药物浓度较高。在地西泮、苯妥英、卡马西平、阿米替林、丙咪嗪和氯普噻吨中毒的情况下，酶水解后得到的结果与对照组相同或更差。在地西泮、多塞平和氯丙嗪非致死性中毒中，用胰蛋白酶消化的样本和对照组的药物血药浓度相似。所有样本均采用XAD-2袋技术用Amberlite XAD-2树脂进行提取。
