通过Amberlite XAD-2柱色谱法从血液中分离药物。

Isolation of drugs from blood by column chromatography on Amberlite XAD-2.

作者信息

Gelbke H P, Grell T H, Schmidt G

出版信息

Arch Toxicol. 1978 Jan 25;39(3):211-7. doi: 10.1007/BF00368229.

Abstract

An investigation was carried out on the isolation of 28 drugs from blood by column chromatography on Amberlite XAD-2. The following substances were added to postmortem blood specimens at concentrations generally of 1--10 microgram/ml: barbital, heptabarbital, hexobarbital, pentobarbital, phenobarbital, phenylbutazone, monocrotophos, amidopyrine, carbromal, diazepam, meprobamate, methaqualone, nitrazepam, phenazetin, chlorpromazine, dibenzepin, diphenhydramine, haloperidol, imipramine, mescaline, methadone, morphine, pentazocine, pethidine, tilidine, triflupromazine, verapamil, N-propylajmalinium bitartrate. The samples were purified by column chromatography on Amberlite XAD-2 and simple solvent extraction and subsequently quantitated by gas chromatography. By systematic variation of the conditions for adsorption of the drugs and desorption from the resin, revoveries of more than 80% after XAD-2 column chromatography could be achieved for most drugs. Thereby it was demonstrated, that in cases of fatal poisoning or emergencies this procedure is a valuable tool for forensic and clinical toxicologists who have to find out the toxic agent by chemical analysis of the blood.

摘要

采用Amberlite XAD - 2柱色谱法从血液中分离28种药物进行了一项研究。以下物质以通常1 - 10微克/毫升的浓度添加到死后血液标本中：巴比妥、庚巴比妥、己巴比妥、戊巴比妥、苯巴比妥、保泰松、久效磷、氨基比林、卡溴脲、地西泮、甲丙氨酯、甲喹酮、硝西泮、非那西丁、氯丙嗪、二苯氮䓬、苯海拉明、氟哌啶醇、丙咪嗪、三甲氧苯乙胺、美沙酮、吗啡、喷他佐辛、哌替啶、替利定、三氟拉嗪、维拉帕米、重酒石酸N - 丙基阿马林。样品通过Amberlite XAD - 2柱色谱法和简单的溶剂萃取进行纯化，随后通过气相色谱法定量。通过系统改变药物在树脂上的吸附和解吸条件，大多数药物在XAD - 2柱色谱后的回收率可达到80%以上。由此证明，在致命中毒或紧急情况的案例中，该方法对于那些必须通过血液化学分析找出有毒物质的法医和临床毒理学家来说是一种有价值的工具。