Blussé van Oud Alblas A, van Furth R
Immunobiology. 1982 Apr;161(3-4):186-92. doi: 10.1016/S0171-2985(82)80073-9.
The controversy as to whether pulmonary macrophages derive from monocytes or from precursor cells in the pulmonary interstitium has been solved by quantitative analysis of 3H-thymidine labeling data on the circulating monocytes and the total population of pulmonary macrophages isolated from mice by lavage and enzyme digestion of lung tissue. It was found that in the normal steady state, during acute inflammatory reactions, and under long-term corticosteroid treatment, pulmonary macrophages derive mainly from circulating monocytes which enter the lungs and become pulmonary macrophages within a few hours. Local division of pulmonary macrophages made only a minor contribution to the maintenance of the population; a dividing precursor cell population located in the interstitium of the lung could not be demonstrated. The few mononuclear phagocytes dividing locally in the lung have most probably arrived recently from the circulation and originate in the bone marrow. These dividing cells already have the characteristics of pulmonary macrophages.
关于肺巨噬细胞是源自单核细胞还是肺间质中的前体细胞这一争议，已通过对循环单核细胞以及通过灌洗和酶消化肺组织从小鼠分离出的肺巨噬细胞总数进行³H-胸腺嘧啶核苷标记数据的定量分析得以解决。结果发现，在正常稳态、急性炎症反应期间以及长期皮质类固醇治疗下，肺巨噬细胞主要源自循环单核细胞，这些单核细胞进入肺部并在数小时内成为肺巨噬细胞。肺巨噬细胞的局部分裂对细胞群体的维持贡献很小；未发现位于肺间质中的分裂前体细胞群体。少数在肺内局部分裂的单核吞噬细胞很可能最近才从循环中抵达，且起源于骨髓。这些分裂细胞已具备肺巨噬细胞的特征。
