Effects of mitogen and liposome phospholipid concentration on the blastogenic response of bovine lymphocytes to phytohemagglutinin.

Lymphocytes incubated with liposomes prior to the addition of phytohemagglutinin (PHA) exhibited a time dependent suppression of blastogenesis which was reversible for phosphatidylcholine (PC):cholesterol (Chol) alpha-tocopherol (alpha-T) (1:0.5:0.5), and PC: alpha-T (1:1) liposomes but not for PC and PC:Chol (1:1) liposomes. Incubation of PHA with lymphocytes prior to the addition of liposomes caused a time dependent, reversible suppression of blastogenesis for PC: alpha-T:Chol (1:0.5:0.5), PC: alpha-T (1:1) and PC:Chol (1:1) liposomes but not reversible for PC liposomes. The effects of phospholipid concentration on the blastogenic response to PHA exhibited a concentration dependent suppression from 1 to 2 mumoles phospholipid/ml which was reversible, to varying degrees, at a concentration of 4 mumoles phospholipid/ml.