Fountain M W, Dees C, Weete J D, Schultz R D
Mol Immunol. 1982 Nov;19(11):1491-8. doi: 10.1016/0161-5890(82)90195-x.
Multilamellar lipid vesicles (MLV) composed of egg yolk lecithin (EYL) suppressed the response of bovine peripheral blood lymphocytes (BPBL) to phytohemagglutinin (PHA). EYL contains 18:1 and 18:2 as the major unsaturated phospholipids. Dioleoyllecithin (DOL; cis 9) MLV did not suppress BPBL blastogenesis. Dilinoeyllecithin (DLL; cis, cis 9, 12) MLV suppressed BPBL blastogenesis. The suppressive effect could be reversed by increasing the MLV DML concentration. The addition of alpha-tocopherol (alpha-T) at 10 mole% into MLV containing DLL reversed blastogenic suppression of BPBL. MLV composed of mixed saturated phospholipids (dimyristoyllecithin and dipalmitoyllecithin) and alpha-T enhanced the BPBL blastogenic response to PHA. BPBL incubated with varying PHA concentrations (11.82-375 mg/ml) and a constant concentration (2 mumoles/ml) of MLV composed of EYL remained suppressed either when PHA and MLV were added simultaneously or when MLV were incubated for 1 hr prior to the addition of PHA. This suggests that alpha-T may act as an immunomodulator in the blastogenic response to PHA. Results suggest that alpha-T reversion of EYL suppression of BPBL blastogenesis may be due to interactions of alpha-T with unsaturated acyl chains in EYL phospholipids.
由蛋黄卵磷脂（EYL）组成的多层脂质体（MLV）抑制了牛外周血淋巴细胞（BPBL）对植物血凝素（PHA）的反应。EYL含有18:1和18:2作为主要的不饱和磷脂。二油酰卵磷脂（DOL；顺式9）MLV不抑制BPBL的增殖。二月桂酰卵磷脂（DLL；顺式，顺式9,12）MLV抑制BPBL的增殖。通过增加MLV的DML浓度可以逆转这种抑制作用。在含有DLL的MLV中加入10摩尔%的α-生育酚（α-T）可逆转BPBL的增殖抑制。由混合饱和磷脂（二肉豆蔻酰卵磷脂和二棕榈酰卵磷脂）和α-T组成的MLV增强了BPBL对PHA的增殖反应。当PHA和MLV同时加入或在加入PHA之前将MLV孵育1小时时，用不同浓度（11.82 - 375 mg/ml）的PHA和恒定浓度（2微摩尔/毫升）的由EYL组成的MLV孵育的BPBL仍然受到抑制。这表明α-T可能在对PHA的增殖反应中作为一种免疫调节剂起作用。结果表明，α-T逆转EYL对BPBL增殖的抑制作用可能是由于α-T与EYL磷脂中的不饱和酰基链相互作用所致。
