通过对应离心淘析法富集对人肿瘤细胞具有细胞毒性的人淋巴细胞。
Enrichment by counterpart centrifugal elutriation of human lymphocytes cytotoxic to human tumour cells.
作者信息
Yasaka T, Wells R J, Mantich N M, Boxer L A, Baehner R L
出版信息
Immunology. 1982 Jul;46(3):613-7.
The enriched fractions of cytotoxic cells responsible for natural killer (NK) activity against both human sarcoma and neuroblastoma (LA-N2) cell lines were readily obtained by countercurrent centrifugal elutriation (CCE). The NK cells were obtained in the larger lymphocyte fractions (fraction 6 +/- 1), having a mean cell volume of 180 u3. The cytotoxic-enriched fraction contained 51% large lymphocytes having cytoplasmic granules. On the other hand, monocytes were purified to greater than 90% and isolated in another fraction (final fraction) and these cells had the lowest NK activity against both human tumour cell lines. However, compared with the lymphocyte fractions, small and large monocytes displayed greater antibody-dependent cellular cytotoxicity (ADCC) activity against human B erythrocytes. These results indicate that NK found to have activity against both tumour cells lines were larger lymphocytes, not small monocytes. Thus, countercurrent centrifugal elutriation (CCE) can provide a sensitive method to obtain enriched fractions of large lymphocytes contained tumoricidal activity against human sarcoma and neuroblastoma cell lines.
通过逆流离心淘析（CCE）很容易获得对人肉瘤和神经母细胞瘤（LA-N2）细胞系具有自然杀伤（NK）活性的细胞毒性细胞富集组分。NK细胞存在于较大的淋巴细胞组分中（第6±1组分），平均细胞体积为180立方微米。细胞毒性富集组分含有51%具有细胞质颗粒的大淋巴细胞。另一方面，单核细胞被纯化至90%以上，并在另一个组分（最终组分）中分离出来，这些细胞对两种人肿瘤细胞系的NK活性最低。然而，与淋巴细胞组分相比，小单核细胞和大单核细胞对人B红细胞显示出更强的抗体依赖性细胞毒性（ADCC）活性。这些结果表明，对两种肿瘤细胞系都具有活性的NK细胞是大淋巴细胞，而非小单核细胞。因此，逆流离心淘析（CCE）可以提供一种灵敏的方法来获得对人肉瘤和神经母细胞瘤细胞系具有杀肿瘤活性的大淋巴细胞富集组分。
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