Kundu S K, Marcus D M, Roelcke D
Immunol Lett. 1982 May;4(5):263-7. doi: 10.1016/0165-2478(82)90049-9.
Anti-Gd and anti-p cold agglutinins exhibit similar serological properties: neuraminidase treatment of erythrocytes greatly reduces their agglutinability by these antibodies and protease treatment enhances their agglutination. We reported previously that an anti-p cold agglutinin was inhibited by sialosyllactoneotetraosylceramide, NeuAc(alpha 2-3)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc-Cer, the most abundant ganglioside of human erythrocytes. We now report that two less abundant gangliosides are more potent inhibitors of this antibody, and of the anti-Gd antibodies, than sialosyllactoneotetraosylceramide. These two gangliosides have the same carbohydrate chain, NeuAc(alpha 2-3)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc(SNH), but they differ in their ceramide moiety. The principal fatty acid of SNH-1 is C16:0, whereas SNH-2 contains a predominance of C22:0, C24:0 and C24:1. No inhibition was produced by the ganglioside, NeuAc(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc-Cer. Another monoclonal cold agglutinin, Sa, which shares some serological properties with anti-Gd cold agglutinins, was not inhibited by any of these gangliosides.
抗-Gd和抗-p冷凝集素表现出相似的血清学特性：用神经氨酸酶处理红细胞可大大降低这些抗体对其的凝集性，而用蛋白酶处理则可增强其凝集作用。我们之前报道过，一种抗-p冷凝集素被唾液酸乳糖新四糖神经酰胺（NeuAc(α2-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc-Cer）抑制，这是人类红细胞中最丰富的神经节苷脂。我们现在报道，两种含量较少的神经节苷脂比唾液酸乳糖新四糖神经酰胺更能有效抑制这种抗体以及抗-Gd抗体。这两种神经节苷脂具有相同的碳水化合物链，即NeuAc(α2-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc(SNH)，但它们的神经酰胺部分不同。SNH-1的主要脂肪酸是C16:0，而SNH-2主要含有C22:0、C24:0和C24:1。神经节苷脂NeuAc(α2-6)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc-Cer没有产生抑制作用。另一种单克隆冷凝集素Sa与抗-Gd冷凝集素具有一些共同的血清学特性，它不受任何这些神经节苷脂的抑制。
