Pederson J E
Invest Ophthalmol Vis Sci. 1982 Aug;23(2):176-80.
Four months after total iridectomy, the aqueous humor of each of six cynomolgus monkeys was perfused at the spontaneous intraocular pressure under urethane anesthesia. The perfusate solution consisted of mock aqueous humor containing trace amounts of 14C-dextran (70,000 mol. wt.). The aqueous was stirred to bathe the ciliary epithelium. Aqueous flow was calculated from the tracer dilution. The aqueous humor was then perfused with bovine serum albumin (35 mg/ml) added to the perfusate. A new aqueous flow value was determined. Aqueous flow during mock aqueous perfusion was 1.28 +/- 0.12 microliters/min and 1.64 +/- 0.12 microliters/min during mock aqueous plus albumin perfusion. From the increase in aqueous flow due to the osmotic effect of the albumin solution (13.2 mm Hg), the osmotic fluid permeability of the ciliary epithelium was calculated to be 0.027 +/- 0.007 microliters/min/mm Hg. This permeability value places the ciliary epithelium in the category of membranes with "leaky" tight junctions. However, the low fluid permeability of the ciliary epithelium suggests a minor role for ultrafiltration in aqueous humor formation in the monkey.
在进行全虹膜切除术后四个月，在氨基甲酸乙酯麻醉下，于自发眼内压状态下对六只食蟹猴的房水进行灌注。灌注液由含有微量14C-葡聚糖（分子量70,000）的模拟房水组成。搅拌房水以使其浸浴睫状体上皮。根据示踪剂稀释法计算房水流量。然后向灌注液中添加牛血清白蛋白（35毫克/毫升）后对房水进行灌注。测定新的房水流量值。模拟房水灌注期间的房水流量为1.28±0.12微升/分钟，模拟房水加白蛋白灌注期间为1.64±0.12微升/分钟。根据白蛋白溶液（13.2毫米汞柱）的渗透作用导致的房水流量增加，计算出睫状体上皮的渗透液通透性为0.027±0.007微升/分钟/毫米汞柱。该通透性值表明睫状体上皮属于具有“渗漏”紧密连接的膜类别。然而，睫状体上皮的低液体通透性表明超滤在猴眼房水生成中起次要作用。
