Kartenbeck J
Cell Biol Int Rep. 1978 Sep;2(5):457-64. doi: 10.1016/0309-1651(78)90097-8.
When coated vesicles isolated from porcine brain are treated with buffers containing 2 M urea and subjected to ultracentrifugation, pellets are obtained which consist of largely smooth-surfaced, i.e. coat-free, vesicles. On dialysis of the urea from the supernatant solution spheroidal particles can be pelleted which are of similar size to the coated vesicles and show the typical polygonal lattice arrays but do not reveal any vesicle membrane profiles. Gel electrophoresis of these membrane-depleted polygonal arrangements demonstrates the predominance, if not exclusive occurrence, of the polypeptide band component with an apparent molecular weight of about 180,000. This suggests that the polygonal coat structure contains only clathrin.
当用含有2M尿素的缓冲液处理从猪脑中分离出的包被小泡，并进行超速离心时，得到的沉淀主要由表面光滑（即无包被）的小泡组成。从上清液中透析去除尿素后，可以沉淀出与包被小泡大小相似的球状颗粒，这些颗粒呈现典型的多边形晶格排列，但没有显示出任何小泡膜的轮廓。对这些去除膜的多边形结构进行凝胶电泳表明，存在一条表观分子量约为180,000的多肽带成分，即使不是唯一存在，也是占主导地位的。这表明多边形包被结构仅包含网格蛋白。
