Pearse B M
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1255-9. doi: 10.1073/pnas.73.4.1255.
Coated vesicles have been purified from brain, adrenal medulla, and a nonsecreting lymphoma cell line. A single major protein species, clathrin, with an apparent molecular weight of 180,000, forms the coat of all these vesicles. Peptide mapping suggests that the amino acid sequence of clathrin is conserved, irrespective of tissue or species studied. Coated vesicles of different sizes are found. The coats are constructed with variable numbers of clathrin subunits, arranged in closed networks of hexagons and pentagons. The amount of clathrin in lymphoma cells suggests that coated vesicles transfer substantial amounts of membrane within cells, not necessarily in association with a secretory process.
包被小泡已从大脑、肾上腺髓质和一种非分泌性淋巴瘤细胞系中纯化出来。一种单一的主要蛋白质——网格蛋白，其表观分子量为180,000，构成了所有这些小泡的包被。肽图谱分析表明，无论所研究的组织或物种如何，网格蛋白的氨基酸序列都是保守的。发现了不同大小的包被小泡。包被由数量可变的网格蛋白亚基构成，这些亚基排列成六边形和五边形的封闭网络。淋巴瘤细胞中网蛋白的含量表明，包被小泡在细胞内转运大量的膜，不一定与分泌过程相关。
