采用高压液相色谱法同时测定剂型中维生素A和维生素D的含量。
Simultaneous determination of vitamins A and D in a dosage forms by high pressure liquid chromatography.
作者信息
Santoro M I, Magalhäes J F, Hackmann E R
出版信息
J Assoc Off Anal Chem. 1982 May;65(3):619-23.
Vitamins A and D were determined simultaneously in oily solutions, ointments, and elixirs, but only vitamin A could be determined in capsules. Samples were saponified with KOH in isopropanol-water, using hydroquinone as antioxidant, and extracted with ether-petroleum ether (1 + 1). After evaporation of solvent, residues were dissolved in isopropanol. Vitamins in these solutions were determined by reverse phase high pressure liquid chromatography, using methanol-water as mobile phase and detection at 254 nm. The reproducibility, using external standards, was 1.6-2.5% and 1.2-3.8% for vitamins A and D, respectively.
在油溶液、软膏剂和酏剂中同时测定维生素A和D，但胶囊剂中只能测定维生素A。样品用氢氧化钾在异丙醇 - 水中皂化，以对苯二酚作为抗氧化剂，并用乙醚 - 石油醚（1 + 1）萃取。溶剂蒸发后，残渣溶于异丙醇。这些溶液中的维生素通过反相高效液相色谱法测定，以甲醇 - 水作为流动相，并在254nm处检测。使用外标法，维生素A和D的重现性分别为1.6 - 2.5%和1.2 - 3.8%。
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