Okazaki M, Hagiwara N, Hara I
J Biochem. 1982 Apr;91(4):1381-9. doi: 10.1093/oxfordjournals.jbchem.a133826.
A determination method for choline-containing phospholipids in serum lipoproteins was established by application of the HPLC method [Hara et al. (1980) J. Biochem. 87, 1863-1865; (1981) 89, 879-887] developed for cholesterol quantitation. The concentration of choline-containing phospholipids in the fraction separated by HPLC using gel permeation columns was determined through colorimetric detection using a commercial enzymatic kit. The optimum conditions for enzymatic reaction in the flow diagram were examined. The concentration calculated from the A500 peak area was found to reflect very precisely the concentration of choline-containing phospholipids in all lipoprotein fractions, and the quantitation of each lipoprotein fraction can be performed with only 10-20 microliters of whole serum. Monitoring the elution patterns by detecting choline-containing phospholipids can give much more information about lipoprotein distributions according to particle size than analyses done by detecting cholesterol.
应用为胆固醇定量开发的高效液相色谱法[原田等人（1980年）《生物化学杂志》87卷，1863 - 1865页；（1981年）89卷，879 - 887页]，建立了血清脂蛋白中含胆碱磷脂的测定方法。使用凝胶渗透柱通过高效液相色谱分离的组分中含胆碱磷脂的浓度，通过使用市售酶试剂盒的比色检测来测定。研究了流程图中酶促反应的最佳条件。发现由A500峰面积计算出的浓度能非常精确地反映所有脂蛋白组分中含胆碱磷脂的浓度，并且仅用10 - 20微升全血清就能对每个脂蛋白组分进行定量。通过检测含胆碱磷脂来监测洗脱模式，相比于检测胆固醇进行的分析，能够提供更多关于脂蛋白按粒径分布的信息。
