Okazaki M, Ohno Y, Hara I
J Biochem. 1981 Mar;89(3):879-87. doi: 10.1093/oxfordjournals.jbchem.a133271.
A simple and rapid method for the quantitation of cholesterol in human serum lipoproteins (VLDL, LDL, HDL2, and HDL3) was developed (1). The content of cholesterol in each lipoprotein fraction was determined by means of a commercial enzymatic reaction kit after separation by high performance liquid chromatography with gel permeation columns. The quantitation of cholesterol could be performed with only 10-20 mu l of serum in less than 50 min by measuring A550 after passing the mixed eluate and enzyme solution through an on-line reactor system of a high-speed chemical derivatization liquid chromatograph. The precision, reproducibility and sensitivity of the quantitation of cholesterol with this method were acceptable, and the values of HDL-cholesterol determined by this method correlated well with those found by the heparin-manganese chloride precipitation method (r=0.958, n=93).
开发了一种简单快速的方法来定量测定人血清脂蛋白（极低密度脂蛋白、低密度脂蛋白、高密度脂蛋白2和高密度脂蛋白3）中的胆固醇(1)。通过使用凝胶渗透柱的高效液相色谱分离后，利用商用酶反应试剂盒测定各脂蛋白组分中的胆固醇含量。通过将混合洗脱液和酶溶液通过高速化学衍生液相色谱的在线反应器系统后测量A550，仅用10 - 20微升血清即可在不到50分钟内完成胆固醇定量。该方法测定胆固醇的精密度、重现性和灵敏度均可接受，用该方法测定的高密度脂蛋白胆固醇值与肝素 - 氯化锰沉淀法测定的值相关性良好（r = 0.958，n = 93）。
