从电鳐胆碱能突触小体中分离突触前质膜组分：一种特定蛋白质的证据
Isolation of a presynaptic plasma membrane fraction from Torpedo cholinergic synaptosomes: evidence for a specific protein.
作者信息
Morel N, Manaranche R, Israël M, Gulik-Krzywicki T
出版信息
J Cell Biol. 1982 May;93(2):349-56. doi: 10.1083/jcb.93.2.349.
Abstract
Synaptosomal plasma membranes were isolated from Torpedo cholinergic synaptosomes which had been purified as previously described or repurified by equilibrium centrifugation. The synaptosomal plasma membrane could be distinguished from postsynaptic membranes by the absence of postsynaptic specific markers (nicotinic AChR) and by its low intramembrane particle complement after freeze fracture. In addition, the presynaptic membrane fraction contained acetylcholinesterase. Gel electrophoresis permitted the identification of a major protein component of the presynaptic membrane fraction which had a molecular weight of 67,000. This protein was not found in postsynaptic membrane or synaptic vesicle fractions. Thus it appeared to be specific to the nerve terminal plasma membrane.
摘要
从电鳐胆碱能突触体中分离出突触体细胞膜，这些突触体已按先前所述方法纯化或通过平衡离心法重新纯化。突触体细胞膜可通过缺乏突触后特异性标记物（烟碱型乙酰胆碱受体）以及冷冻断裂后其膜内颗粒数量较少与突触后膜区分开来。此外，突触前膜部分含有乙酰胆碱酯酶。凝胶电泳能够鉴定出突触前膜部分的一种主要蛋白质成分，其分子量为67,000。这种蛋白质在突触后膜或突触小泡部分未被发现。因此，它似乎是神经末梢细胞膜所特有的。
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