电鳐电器官突触体的大规模纯化
Large-scale purification of Torpedo electric organ synaptosomes.
作者信息
Israël M, Lazereg S, Lesbats B, Manaranche R, Morel N
出版信息
J Neurochem. 1985 Apr;44(4):1107-10. doi: 10.1111/j.1471-4159.1985.tb08731.x.
Abstract
A procedure for the large-scale purification of Torpedo electric organ synaptosomes is described. The synaptosomal fraction obtained is very pure as judged from biochemical and morphological data. In addition, acetylcholine (ACh) release was demonstrated after KCl depolarization of synaptosomes in the presence of calcium. Two hundred grams of electric organ can be fractionated in a single run, allowing biochemical studies on presynaptic membrane constituents.
摘要
本文描述了一种用于大规模纯化电鳐电器官突触体的方法。从生化和形态学数据判断，所获得的突触体部分非常纯净。此外，在存在钙的情况下，突触体经氯化钾去极化后可证明有乙酰胆碱（ACh）释放。单次运行可对200克电器官进行分级分离，从而能够对突触前膜成分进行生化研究。
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