de Ziegler D, Gurpide E
J Clin Endocrinol Metab. 1982 Sep;55(3):511-5. doi: 10.1210/jcem-55-3-511.
Human decidual tissue has been reported to secrete human PRL in vitro. Decidual scraped from fetal membranes delivered at term was treated with collagenase, and cultures of the dispersed cells were examined 7 days after plating. These cultures were fibroblastic in appearance and secreted insignificant amounts of PRL to the medium (less than 12 ng/ml). However, PRL-producing cells could be selected by taking advantage of the slowness of their attachment to the plastic dishes. Cultures of cells that did not attach during the first 48 h after cell dispersion produced, after attachment, about 100 micrograms PRL/mg DNA in 2 days. This rate is much higher than rates observed during batch incubations or superfusions of minced decidual preparations (approximately 0.2-0.3 micrograms PRL/mg DNA.day). PRL production rates declined after the seventh day of culture, probably as a consequence of overgrowth of cells that did not secrete PRL. Cultures enriched in PRL-secreting cells may be used to study the regulation of decidual production of PRL and other biochemical processes of the endometrium affected by decidualization.
据报道，人蜕膜组织在体外可分泌人催乳素。从足月分娩的胎膜刮取的蜕膜用胶原酶处理，接种后7天检查分散细胞的培养物。这些培养物外观呈成纤维细胞样，向培养基中分泌的催乳素量极少（低于12 ng/ml）。然而，可利用催乳素产生细胞附着于塑料培养皿的速度较慢这一特点来筛选它们。细胞分散后最初48小时内未附着的细胞培养物，在附着后2天内每毫克DNA可产生约100微克催乳素。该速率远高于在切碎的蜕膜制剂分批培养或灌流过程中观察到的速率（约0.2 - 0.3微克催乳素/毫克DNA·天）。培养第7天后催乳素产生速率下降，可能是由于不分泌催乳素的细胞过度生长所致。富含催乳素分泌细胞的培养物可用于研究蜕膜催乳素产生的调节以及受蜕膜化影响的子宫内膜的其他生化过程。
