Hung C T, Selkirk A B, Taylor R B
J Clin Hosp Pharm. 1982 Mar;7(1):17-23. doi: 10.1111/j.1365-2710.1982.tb00903.x.
A specific qualitative chromatographic assay method is described for 5-hydroxymethylfurfuraldehyde (5HMF) in degraded D-glucose solutions incorporating an internal standard. Comparison is made between the chromatographic method and the official direct ultraviolet absorption measurements at 284 nm for solutions of D-glucose degraded to different extents. Hospital samples of autoclaved Dextrose Injection, 5% w/v, are also examined by both methods and it is demonstrated that quite different results can be obtained depending upon the conditions of measurement. It is demonstrated that the direct absorption method does not provide an adequate limit test for compounds related to 5HMF.
描述了一种用于测定含有内标物的降解 D - 葡萄糖溶液中 5 - 羟甲基糠醛（5HMF）的特定定性色谱分析方法。对不同程度降解的 D - 葡萄糖溶液，将该色谱方法与 284 nm 处的官方直接紫外吸收测量法进行了比较。还使用这两种方法对 5% w/v 的高压灭菌葡萄糖注射液的医院样本进行了检测，结果表明，根据测量条件的不同，可得到截然不同的结果。结果表明，直接吸收法无法为与 5HMF 相关的化合物提供充分的限度检测。
