Eckberg W R, Metz C B
J Exp Zool. 1982 May 20;221(1):101-5. doi: 10.1002/jez.1402210113.
Antisperm antibody fragments (IFab) block sea urchin fertilization by inhibiting the acrosome reaction and consequently sperm-egg attachment. We describe here a 68,000 dalton MW glycoprotein which neutralizes the fertilization-inhibiting action of IFab. This glycoprotein is a minor component is SDS-polyacrylamide gels of sperm membranes, but is greatly enriched in lithium diiodosalicylate extracts of the membranes. Final purification of the antigen was accomplished by elution from preparative SDS-polyacrylamide gels. This glycoprotein is evidently the sea urchin sperm receptor which interacts wih the egg jelly coat and acts as a "trigger" for the acrosome reaction.
抗精子抗体片段（IFab）通过抑制顶体反应从而阻止精子与卵子的附着，进而阻断海胆受精过程。我们在此描述了一种分子量为68,000道尔顿的糖蛋白，它能中和IFab的受精抑制作用。这种糖蛋白是精子膜SDS - 聚丙烯酰胺凝胶中的次要成分，但在膜的二碘水杨酸锂提取物中大量富集。通过从制备性SDS - 聚丙烯酰胺凝胶上洗脱实现了该抗原的最终纯化。这种糖蛋白显然是海胆精子受体，它与卵黄膜相互作用，并作为顶体反应的“触发因素”。
