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Detection of H-2 antigens on 8-cell mouse embryos.

作者信息

Cozad K M, Warner C M

出版信息

J Exp Zool. 1982 Jun 10;221(2):213-7. doi: 10.1002/jez.1402210212.

Abstract

H-2 antigens were detected on 80-cell mouse embryos of the b, k, and d haplotypes, but not on 8-cell embryos of the a haplotype. The technique used for detection of H-2 antigens on the embryos was a highly sensitive, automated, complement-dependent cytotoxicity assay, based on the principle that live embryos will incorporate 3H-thymidine into DNA, but embryos killed with antiserum and complement will not. The amount of H-2 present on 8-cell embryos is apparently less than that present on blastocysts. A possible function of H-2 antigens in development is proposed.

摘要

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