输卵管包裹的小鼠二细胞胚胎低温（4℃）运输的研究。

Study of low-temperature (4 degrees C) transport of mouse two-cell embryos enclosed in oviducts.

作者信息

Kamimura Eikichi, Nakashima Tatsuyuki, Ogawa Mami, Ohwada Kazuo, Nakagata Naomi

机构信息

Laboratory Animal Center, Yamagata University School of Medicine, 2-2-2 Iidanishi, Yamagata, 990-9585, Japan.

出版信息

Comp Med. 2003 Aug;53(4):393-6.

PMID:14524415

Abstract

PURPOSE

We examined usefulness of a mouse embryo transportation system for low-temperature transport of oviducts containing mouse two-cell embryos.

METHODS

Oviducts containing two-cell mouse embryos were stored at 4 degrees C for 36 h. After that, embryos were collected and cultured for 96 h in Potassium Simplex Optimized Medium (KSOM) medium and evaluated for their rate of development to hatched blastocysts. Embryos were transferred to recipients, and the rate of survival to live young was investigated. The oviducts were then transported from Yamagata to Kumamoto (distance of approx. 1,000 km). At the destination, embryos were implanted in recipient dams and were studied to evaluate their survival to live young.

RESULTS

After preservation for 36 h at 4 degrees C, 68.3% of two-cell embryos developed to hatched blastocysts. As a result of transplanting 546 embryos into 25 recipients, 109 normal live young mice were obtained; the rate of development was 20.0%. Results of oviduct transport from Yamagata to Kumamoto indicated that 30.2% of transplanted embryos developed to live young.

CONCLUSION

Low-temperature transport of two-cell embryos in oviducts is useful as a method of shipping mouse embryos between institutes.

摘要

目的

我们研究了一种小鼠胚胎运输系统对于低温运输含有小鼠二细胞胚胎的输卵管的实用性。

方法

将含有二细胞小鼠胚胎的输卵管在4℃下保存36小时。之后，收集胚胎并在优化的单纯钾培养基（KSOM）中培养96小时，评估其发育至孵化囊胚的比率。将胚胎移植到受体中，研究其存活至幼崽出生的比率。然后将输卵管从山形运送到熊本（距离约1000公里）。在目的地，将胚胎植入受体母鼠体内，并研究评估其存活至幼崽出生的情况。