Heinitz M L
J Pharm Sci. 1982 Jun;71(6):656-8. doi: 10.1002/jps.2600710612.
A convenient high-performance liquid chromatographic method for the quantitative determination of cyclobenzaprine hydrochloride in tablets is described. Samples were dissolved in 0.05 N sulfuric acid and diluted with methanol. The cyclobenzaprine hydrochloride was chromatographed using an octylsilane column and the eluent acetonitrile-0.6% dibasic potassium phosphate aqueous buffer (pH 3.0) (75:25) at a flow rate of 1.5 ml/min. Naphazoline hydrochloride was used as an internal standard. The UV detector response at 254 nm was linear for cyclobenzaprine hydrochloride in the 0.005-0.03 mg/ml range under conditions of the analysis.
本文描述了一种简便的高效液相色谱法，用于定量测定片剂中的盐酸环苯扎林。样品溶解于0.05N硫酸中，并用甲醇稀释。使用辛基硅烷柱对盐酸环苯扎林进行色谱分析，流动相为乙腈-0.6%磷酸氢二钾水溶液缓冲液（pH 3.0）（75:25），流速为1.5 ml/min。盐酸萘甲唑啉用作内标。在该分析条件下，盐酸环苯扎林在0.005 - 0.03 mg/ml范围内，254 nm处的紫外检测器响应呈线性。
