Espevik T, Lamvik M K, Sunde A, Eik-Nes K B
J Reprod Fertil. 1982 Jul;65(2):489-95. doi: 10.1530/jrf.0.0650489.
The toxicity of different metals on isolated Sertoli cells grown in culture has been investigated. Methyl mercury (CH3HgCl) and mercury chloride (HgCl2) were more toxic than cadmium (CdCl2) which was slightly more toxic than arsenic (As2O3). Isolated peritubular cells and Sertoli cells were equally sensitive to cadmium. Cadmium reduced the Sertoli cell survival over the concentration range of 1--10 microM. Freeze-etch electron microscopy of cadmium-exposed Sertoli cells revealed circular areas of average diameter 500 nm devoid of intramembrane particles in the nuclear membrane, and general signs of degeneration such as vesiculation of the plasma membrane and intramembrane particle aggregation. However, cadmium did not dissolve junctional complexes between Sertoli cells. Isolated Sertoli cells were protected against cadmium-induced damage when the cells were preincubated for 48 h with selenium, zinc or low doses of cadmium. Preincubation with cobalt, FSH, testosterone or oestradiol did not protect against cadmium-induced damage. Cadmium bound to metallothionein had no toxic effects on isolated Sertoli cells.
研究了不同金属对培养的分离支持细胞的毒性。甲基汞（CH3HgCl）和氯化汞（HgCl2）的毒性比镉（CdCl2）更大，而镉的毒性比三氧化二砷（As2O3）略高。分离的睾丸间质细胞和支持细胞对镉同样敏感。在1-10微摩尔的浓度范围内，镉会降低支持细胞的存活率。对暴露于镉的支持细胞进行冷冻蚀刻电子显微镜观察发现，核膜上平均直径为500纳米的圆形区域没有膜内颗粒，并且存在细胞膜泡化和膜内颗粒聚集等一般退化迹象。然而，镉不会溶解支持细胞之间的连接复合体。当分离的支持细胞与硒、锌或低剂量的镉预孵育48小时时，可免受镉诱导的损伤。与钴、促卵泡激素、睾酮或雌二醇预孵育不能防止镉诱导的损伤。与金属硫蛋白结合的镉对分离的支持细胞没有毒性作用。
