Hew K W, Heath G L, Jiwa A H, Welsh M J
Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor 48109.
Biol Reprod. 1993 Oct;49(4):840-9. doi: 10.1095/biolreprod49.4.840.
Ten-week-old male Sprague-Dawley rats were injected i.p. with cadmium chloride solution in a single dose of 0 or 1.0 mg/kg BW. At 4, 24, 48, and 72 h after injection, testes of the animals were collected, detunicated, and fixed in 10% formalin. Individual seminiferous tubules were isolated and the stages of the cycle of the seminiferous epithelium were determined through use of transillumination under a stereomicroscope. The tubules were stained with rhodamine-phalloidin, mounted on glass slides, and examined via a confocal microscope. This cadmium dose did not cause visible vascular lesion in the testes. The cadmium treatment resulted in changes in the pattern produced by microfilaments in the basal region of Sertoli cells. The observed change in basal Sertoli cell microfilaments consisted of fragmentation of the microfilament bundles as compared to those in seminiferous tubules from control animals. This apparent lesion was first observed in stages VIII through XI at 24 h after the cadmium exposure. As the time after exposure increased, the lesion within a stage occurred with increasing severity, and later stages of the cycle of the seminiferous epithelium were also affected. At 48 h after exposure, disorganization of microfilament bundles was seen in stages VIII through XIII/XIV. At 72 h after exposure, severe fragmentation of microfilament bundles was observed from stage VIII through stages II/III. The microfilament bundles in several stages prior to stage VIII remained unaffected. No change was observed in the microfilaments of peritubular cells. At 4 h after exposure, testes showed no change in the organization of microfilament bundles at the basal region of Sertoli cells or microfilaments in the peritubular cells. We conclude that a single cadmium chloride dose of 1 mg/kg results in the disruption of basal Sertoli cell microfilament bundles in the rat seminiferous epithelium, and that the action of cadmium is cell-specific and stage-specific.
将10周龄的雄性斯普拉格-道利大鼠腹腔注射氯化镉溶液，单剂量分别为0或1.0毫克/千克体重。在注射后4、24、48和72小时，收集动物的睾丸，去除被膜，固定于10%福尔马林中。分离出单个生精小管，通过在体视显微镜下利用透照法确定生精上皮周期的阶段。将小管用罗丹明-鬼笔环肽染色，置于载玻片上，通过共聚焦显微镜检查。该镉剂量未导致睾丸出现可见的血管病变。镉处理导致支持细胞基部区域微丝产生的模式发生变化。与对照动物生精小管中的微丝束相比，观察到的支持细胞基部微丝变化包括微丝束断裂。这种明显的损伤在镉暴露后24小时首次在VIII至XI期观察到。随着暴露后时间的增加，一个阶段内的损伤严重程度增加，生精上皮周期的后期阶段也受到影响。暴露后48小时，在VIII至XIII/XIV期可见微丝束紊乱。暴露后72小时，从VIII期到II/III期观察到微丝束严重断裂。VIII期之前的几个阶段的微丝束未受影响。在周细胞的微丝中未观察到变化。暴露后4小时，睾丸在支持细胞基部区域的微丝束组织或周细胞中的微丝方面未显示变化。我们得出结论，1毫克/千克的单剂量氯化镉导致大鼠生精上皮中支持细胞基部微丝束破坏，并且镉的作用具有细胞特异性和阶段特异性。
