两种电泳法和两种柱色谱法测定血清肌酸激酶同工酶MB活性的相对优缺点。
Relative merits of two electrophoretic and two column-chromatographic kets for determining serum creatine kinase isoenzyme MB activity.
作者信息
Hamlin C, Ackerman E
出版信息
Clin Chem. 1978 Nov;24(11):2013-7.
Prospective analysis (by use of commercially available kits) of 339 validated admission specimens from patients consecutively admitted to a cardiac monitoring unit revealed sensitivities of 68 and 63% for two electrophoretic assays (Bioware and Helena) and up to 73 and 77% for two chromatographic assays (Roche and Worthington). Specificities were 94.8, 96,6, 90.2, and 89.7% and efficiencies were 90.2, 90.7, 87.0, and 87.0, respectively. Although these results differ from early reports, they agree well with several recent studies. However, 86 (25%) of the specimens gave at least one discrepant result based on clinical evaluation of the patients, and 68 (20%) gave at least one discrepant result between methods.
对连续入住心脏监护病房的患者的339份经验证的入院样本进行前瞻性分析（使用市售试剂盒），结果显示两种电泳分析方法（Bioware和Helena）的灵敏度分别为68%和63%，两种色谱分析方法（罗氏和沃辛顿）的灵敏度高达73%和77%。特异性分别为94.8%、96.6%、90.2%和89.7%，效率分别为90.2%、90.7%、87.0%和87.0%。尽管这些结果与早期报告不同，但与最近的几项研究结果非常吻合。然而，根据对患者的临床评估，86份（25%）样本至少给出了一个不一致的结果，68份（20%）样本在不同方法之间至少给出了一个不一致的结果。
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