Venkatesan K, Bharadwaj V P, Sritharan V, Girdhar B K
Lepr India. 1982 Jan;54(1):95-9.
With an aim to evaluate the metabolic disposition of Dapsone (DDS) in protein malnutrition, a study was undertaken to determine DDS content of blood and tissues of protein deficient mice in comparison to the levels in ad-libitum and pair-fed mice. Three groups of Rockfeller's strain mice of same sex (roughly weighing 25 gm) were fed on low protein diet and high protein diet (for pair-fed and ad-libitum groups). After one month feeding DDS was administered by oral intubation daily for 7 days at a dose of 0.01 mg per g body weight. On 7th day animals were sacrificed 3 hours after DDS administration. Heparinised blood was collected from ocular plexus. Tissues (liver and kidney) were taken out for DDS estimations. DDS levels were determined by the method of Simpson (1949) and tissue protein content was assayed by the method of Lowry et al (1951). Hypoproteinemic mice presented higher values for blood and tissue DDS as compared to their pair-fed and ad-libitum fed groups. Our observations indicate that in hypoproteinemic mice, on account of the possible diminished binding of DDS to plasma albumin, the concentration of free DDS in blood and in tissues is higher.
为了评估氨苯砜（DDS）在蛋白质营养不良中的代谢情况，开展了一项研究，以确定蛋白质缺乏小鼠血液和组织中的DDS含量，并与自由采食和配对喂养小鼠的水平进行比较。将三组相同性别的洛克菲勒品系小鼠（体重约25克）分别喂以低蛋白饮食和高蛋白饮食（用于配对喂养组和自由采食组）。喂养一个月后，每天通过口服插管以每克体重0.01毫克的剂量给予DDS，持续7天。在第7天，给药DDS 3小时后处死动物。从眼丛采集肝素化血液。取出组织（肝脏和肾脏）进行DDS测定。DDS水平采用辛普森（1949年）的方法测定，组织蛋白含量采用洛瑞等人（1951年）的方法测定。与配对喂养组和自由采食组相比，低蛋白血症小鼠血液和组织中的DDS值更高。我们的观察结果表明，在低蛋白血症小鼠中，由于DDS与血浆白蛋白的结合可能减少，血液和组织中游离DDS的浓度更高。
