Rothaul A L, Broadley K J
J Pharmacol Methods. 1982 Mar;7(2):91-103. doi: 10.1016/0160-5402(82)90021-3.
The release of coronary vasodilator mediator(s) from guinea pig isolated hearts was demonstrated by a technique employing donor and recipient hearts perfused in series. Vasodilator mediator release followed the positive inotropic, chronotropic, and coronary vasodilator responses of donor hearts to isoprenaline and their exposure to hypoxia. Isoprenaline-induced coronary vasodilatation of donor hearts was accompanied by a fall in perfusate pO2, indicative of a raised myocardial oxygen consumption. Measurement of perfusate pO2 levels showed that the regassing procedure was adequate and the fall in pO2 level no longer occurred after isoprenaline. However, at high resolution, small changes in pO2 could still be detected by these were not sufficient to induce vasodilatation of the recipient. The technique was adapted to enable simultaneous measurement of perfusate pO2 and release of vasodilator mediator following administration of isoprenaline. The value of this in examining the relationship between myocardial oxygen consumption and vasodilator mediator release is discussed.
通过一种使用串联灌注供体心脏和受体心脏的技术，证明了豚鼠离体心脏释放冠状动脉血管舒张介质。血管舒张介质的释放伴随着供体心脏对异丙肾上腺素的正性肌力、变时性和冠状动脉血管舒张反应以及它们暴露于缺氧环境。异丙肾上腺素诱导的供体心脏冠状动脉血管舒张伴随着灌注液pO2的下降，这表明心肌耗氧量增加。灌注液pO2水平的测量表明，再通气程序是充分的，异丙肾上腺素后pO2水平不再下降。然而，在高分辨率下，仍可检测到pO2的微小变化，但这些变化不足以诱导受体血管舒张。该技术经过改进，能够在给予异丙肾上腺素后同时测量灌注液pO2和血管舒张介质的释放。讨论了这在研究心肌耗氧量与血管舒张介质释放之间关系方面的价值。
