源自年轻和老年供体人皮肤成纤维细胞的单个细胞杂种的复制潜力。

Replicative potential of individual cell hybrids derived from young and old donor human skin fibroblasts.

作者信息

Muggleton-Harris A L, Aroian M A

出版信息

Somatic Cell Genet. 1982 Jan;8(1):41-50. doi: 10.1007/BF01538649.

Abstract

Neonatal, adult, and aged donor human skin fibroblast cell cultures have been characterized for the population doubling potential and in vitro-in vivo relationship. Hybrid cells derived from individual whole-cell fusions of replicating GRC 387 (aged) and CSC 303 (neonatal) cells demonstrated that an intermediate mode of replication between that of the two parental cell lines occurred; therefore the longevity of the aged fibroblast cells was enhanced. The GRC 387 cells have an extended post-replicative phase-out period in comparison with the CSC 303 cells, and the experimental hybrids demonstrated a 20-25% increase of the period over that of the GRC 387 cells.

摘要

已对新生儿、成人和老年供体的人皮肤成纤维细胞培养物的群体倍增潜力以及体外-体内关系进行了表征。从复制型GRC 387（老年）细胞和CSC 303（新生儿）细胞的单个全细胞融合中获得的杂交细胞表明，在两个亲代细胞系之间出现了一种中间复制模式；因此，老年成纤维细胞的寿命得到了延长。与CSC 303细胞相比，GRC 387细胞具有延长的复制后淘汰期，实验杂交细胞显示该时期比GRC 387细胞增加了20%-25%。