Titration of antiserum to South American rattlesnake (Crotalus durissus terrificus) venom by measuring inhibition of phospholipase A2 activity.

Horse antiserum to the venom of Crotalus durissus terrificus, A South American rattlesnake, inhibits the phospholipase activity of the crude venom. There is a close relationship between this inhibitory property and the neutralizing potency of the antiserum in vivo. This may provide the basis for a rigorous standardization of anticrotalid venom in vitro.