Dos-Santos M C, Yamaguchi I K, Caricatti C P, Higashi H G, Dias-da-Silva W
Departamento de Immunologia, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1989;22(4):509-12.
Equines (2 horses and 2 donkeys) immunized with whole Crotalus durissus terrificus venom or its phospholipase A2 component either presented an increased survival time determined 3 days after challenge or were totally resistant to a challenging lethal dose of 200 mg crude venom 270 days after the initial immunization or 90 days after the last booster injection. The resistance was demonstrable on the basis of a good correlation with antibody titers determined by the ELISA method but not with the flocculation and neutralization assays. Since phospholipase A2 is essentially nontoxic, it can be used as a substitute for whole venom for the production of commercial antisera and as an immunizing agent in prophylactic programs.
用杜氏响尾蛇（Crotalus durissus terrificus）全毒或其磷脂酶A2组分免疫的马（2匹马和2头驴），在攻击后3天测定的存活时间延长，或者在初次免疫后270天或最后一次加强注射后90天，对200毫克粗毒的致死攻击剂量完全有抵抗力。这种抵抗力与通过ELISA方法测定的抗体滴度有良好的相关性，但与絮凝和中和试验无关。由于磷脂酶A2基本无毒，它可作为全毒的替代品用于生产商业抗血清，并可作为预防性方案中的免疫剂。
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