Stevens R H, Cole D A
Toxicol Lett. 1982 May;11(3-4):299-303. doi: 10.1016/0378-4274(82)90164-3.
Studies were undertaken to determine whether urine collected from rats during the 24 h following exposure to the colon carcinogen 1,2-dimethylhydrazine (DMH) contained substances which would induce specific antitumor immunity. The evaluation for such components was carried out by administering the test and control urine to young male adult Fisher F344 inbred rats and then establishing the animals' antitumor cell-mediated immunity (CMI) at a 14-day post-exposure interval. The CMI was measured by determining the injury and destruction inflicted upon cultured X-ray-induced rat small bowel adenocarcinoma target cells by peripheral blood lymphoid cells obtained from the animals exposed to the urine. A significantly increased CMI was found to be induced by the urine collected from the chemically exposed rats, thus suggesting the presence of mutagenic/carcinogenic components. In addition, exposure through the levels of CMI that were induced, with an approximate threshold detection limit of 100 microgram (1.7 mumol)/kg body weight (100 ppb) to the chemical. These preliminary findings suggest that such immune measurements might serve as a basis for the development of a rapid and inexpensive bioassay for monitoring population exposures to carcinogenic substances.
开展了多项研究，以确定在暴露于结肠致癌物1,2 - 二甲基肼（DMH）后的24小时内从大鼠收集的尿液中是否含有能诱导特异性抗肿瘤免疫的物质。通过将测试尿液和对照尿液给予年轻雄性成年Fisher F344近交系大鼠，然后在暴露后14天的间隔时间建立动物的抗肿瘤细胞介导免疫（CMI），来评估此类成分。通过测定从暴露于尿液的动物获得的外周血淋巴细胞对培养的X射线诱导的大鼠小肠腺癌靶细胞造成的损伤和破坏来测量CMI。发现化学暴露大鼠收集的尿液可诱导CMI显著增加，这表明存在诱变/致癌成分。此外，通过诱导的CMI水平，以约100微克（1.7微摩尔）/千克体重（100 ppb）的近似阈值检测限接触该化学物质。这些初步发现表明，此类免疫测量可能为开发一种快速且廉价的生物测定方法奠定基础，用于监测人群对致癌物质的暴露情况。
