Alderman J A, Walden R, Schiller C M
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1983;76(2):383-9. doi: 10.1016/0742-8413(83)90094-4.
Pregnant F-344 rats were exposed by intubation to a single dose (35 mg/kg) of 1,2-dimethylhydrazine dihydrochloride or to an acetate buffer on day 14 of gestation. A detailed examination of the effects of this dose of 1,2-dimethylhydrazine on brush border enzymes in the offspring was performed. Changes in the liver and colon levels of the alpha-glycerophosphate and malate/aspartate substrate cycle enzymes were measured during the development; at days 17 and 20 of gestation and at 2, 6, 13, 20, 27, 55, 110 days and 1 year after birth. It is concluded that metabolic energy enzymes, glutamate oxaloacetate transaminase and malate dehydrogenase, are more sensitive to 1,2-dimethylhydrazine treatment than are the brush border hydrolases or NAD- and Fp-linked alpha-glycerophosphate dehydrogenases.
妊娠第14天，将怀孕的F-344大鼠通过插管给予单剂量（35毫克/千克）的二盐酸1,2 - 二甲基肼或醋酸盐缓冲液。对该剂量的1,2 - 二甲基肼对后代刷状缘酶的影响进行了详细检查。在发育过程中，即妊娠第17天和20天以及出生后2天、6天、13天、20天、27天、55天、110天和1年时，测量肝脏和结肠中α-甘油磷酸和苹果酸/天冬氨酸底物循环酶水平的变化。得出的结论是，代谢能量酶、谷氨酸草酰乙酸转氨酶和苹果酸脱氢酶比刷状缘水解酶或NAD和Fp连接的α-甘油磷酸脱氢酶对1,2 - 二甲基肼处理更敏感。
