Fabiani A
Zentralbl Neurochir. 1982;43(1):35-42.
The stability of lysosomes is a function of osmotic pressure, temperature and time. Detailed studies have shown that the release and availability of lysosomal enzymes changes according to the degree of membrane permeability. This paper describes a modification of the controlled temperature and osmotic freezing-sectioning technique for characterization of lysosomal stability in brain tumors. The method is based on the enzyme measurements of released acid phosphatase from sections of unfixed tissue under appropriate physical conditions. The results suggest the possibility of a relation between lysosomal stages of activation, enzyme latency and brain tumor malignancy.
溶酶体的稳定性是渗透压、温度和时间的函数。详细研究表明，溶酶体酶的释放和可用性会根据膜通透性的程度而变化。本文描述了一种用于表征脑肿瘤中溶酶体稳定性的控温及渗透压冷冻切片技术的改进方法。该方法基于在适当物理条件下对未固定组织切片中释放的酸性磷酸酶进行酶活性测定。结果提示了溶酶体激活阶段、酶潜伏性与脑肿瘤恶性程度之间存在关联的可能性。
