Iwamasa T, Hamada T, Fukuda S, Ninomiya N, Takeuchi T
Acta Pathol Jpn. 1982 May;32(3):461-71.
Glycogen extracted from rat ascites hepatoma AH 13 cells was compared with that from adult rat liver, adult rat muscle, and fetal rat liver 3 days before delivery, by electron microscopic, biochemical and physicochemical techniques. The ascites hepatoma AH 13 glycogen revealed two major different types of glycogen macromolecules by sucrose discontinuous density gradient centrifugation. The glycogen of the light fraction was very similar to rat muscle glycogen macromolecules in its size and shape except that it exhibited a stronger Cotton effect on measurement of the optical rotatory dispersion and circular dichroism than the muscle glycogen. Furthermore, this light material different from normal muscle glycogen. The other glycogen which also included very small amounts of irregular particles of glycogen macromolecules was contained in the heavy fraction was very similar to the adult rat liver glycogen macromolecules which did not display the Cotton effect. Electron micrographs showing the ultrastructure of the glycogen particles extracted from the AH 13 cells by negative staining revealed a variety of size and shape, and they contained two types of glycogen macromolecules with the smaller muscle types and larger adult type of liver glycogen macromolecules. The glycogen macromolecules also formed varied irregular structures in conformation.
采用电子显微镜、生化及物理化学技术，对从大鼠腹水肝癌AH 13细胞中提取的糖原与成年大鼠肝脏、成年大鼠肌肉以及分娩前3天的胎鼠肝脏中的糖原进行了比较。通过蔗糖不连续密度梯度离心法，腹水肝癌AH 13糖原显示出两种主要不同类型的糖原大分子。轻组分中的糖原在大小和形状上与大鼠肌肉糖原大分子非常相似，只是在旋光色散和圆二色性测量中，它表现出比肌肉糖原更强的科顿效应。此外，这种轻物质不同于正常肌肉糖原。重组分中包含的另一种糖原，其中也含有极少量不规则的糖原大分子颗粒，与未显示科顿效应的成年大鼠肝脏糖原大分子非常相似。通过负染色显示从AH 13细胞中提取的糖原颗粒超微结构的电子显微镜照片显示，其大小和形状各异，并且它们包含两种类型的糖原大分子，即较小的肌肉型和较大的成年肝脏型糖原大分子。糖原大分子在构象上也形成了各种不规则结构。
